Fig 2: Methylation values obtained from our CRC discovery set and TCGA validation set. Genomic organization of SPOCK1 (A) and SLC6A3 (B), including the localization of exons and CGIs. Mean ß values, resulting from the average of the samples (normal and tumour) of each probe mapping on the altered CGIs associated with SPOCK1 (A) and SLC6A3 (B). When more than one CGI is shown, the altered one is enclosed in a yellow box.

Fig 3: Differential gene expression analysis between tumour and normal samples. Box plot fold change values of GRIA4 (A), VIPR2 (B), SPOCK1 (C) and SLC6A3 (D) for CRC and control samples. *** indicates p value < 0.0001.

Fig 4: Protein expression level for each paired sample. (A) Representative blots of GluR4, VIPR2, SPOCK1 and SLC6A3 protein expression in 10 CRC paired tissues samples. NaK ATPase was used as loading control. (B) Box plots of GluR4, VIPR2, SPOCK1 and SLC6A3 expression. Asterisks indicate statistically significant differences (* p value < 0.05, ** p value < 0.01, *** p value < 0.001). For the SPOCK1 expression study, there was not enough protein lysate for samples 19 and 21.